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Addgene

Pdx1-RFP puro (DM#235)
(Plasmid #15085)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS II
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pdx1 promoter
  • Alt name
    Pdx1
  • Alt name
    pGDRF-puro
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
  • Tag / Fusion Protein
    • RFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed to sort out Pdx1 expressing cells by puromycin resistancy. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pdx1-RFP puro (DM#235) was a gift from Douglas Melton (Addgene plasmid # 15085 ; http://n2t.net/addgene:15085 ; RRID:Addgene_15085)

  • For your References section:

    Insulin staining of ES cell progeny from insulin uptake. Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA. Science. 2003 Jan 17. 299(5605):363. 10.1126/science.1077838 PubMed 12532008
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