pBS mNotch1-IC (DM#270)
(Plasmid #15079)

• Depositing Lab: Douglas Melton
• Publication: Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.

Backbone

• Vector backbone: pBluescript II KS +
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch1-IC
• Alt name: Notch1
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: K1750A
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes an intracellular fragment of mouse Notch1 (amino acids 1749–2293, lacking the C-terminal PEST domain).