pSP64TNE Xnr4 (DM#174)
(Plasmid
#15078)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP64TNE
- Backbone size w/o insert (bp) 3260
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXnr4
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Alt nameXenopus nodal-related 4
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SpeciesX. laevis (frog)
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Insert Size (bp)1300
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Entrez Genexnr4 (a.k.a. xnr4, nr4, nodal4-A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For use in mRNA microinjection experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64TNE Xnr4 (DM#174) was a gift from Douglas Melton (Addgene plasmid # 15078 ; http://n2t.net/addgene:15078 ; RRID:Addgene_15078) -
For your References section:
Xnr4: a Xenopus nodal-related gene expressed in the Spemann organizer. Joseph EM, Melton DA. Dev Biol. 1997 Apr 15. 184(2):367-72. 10.1006/dbio.1997.8510 PubMed 9133442
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/95/49/c1752628-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)