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Addgene

pSP64TNE Xnr4 (DM#174)
(Plasmid #15078)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64TNE
  • Backbone size w/o insert (bp) 3260
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xnr4
  • Alt name
    Xenopus nodal-related 4
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1300
  • Entrez Gene
    xnr4 (a.k.a. xnr4, nr4, nodal4-A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use in mRNA microinjection experiments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64TNE Xnr4 (DM#174) was a gift from Douglas Melton (Addgene plasmid # 15078 ; http://n2t.net/addgene:15078 ; RRID:Addgene_15078)

  • For your References section:

    Xnr4: a Xenopus nodal-related gene expressed in the Spemann organizer. Joseph EM, Melton DA. Dev Biol. 1997 Apr 15. 184(2):367-72. 10.1006/dbio.1997.8510 PubMed 9133442
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