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pSP64T3 BMP2 pro dnmVg1 (DM#182)
(Plasmid #15074)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T3
  • Backbone manufacturer
    Modified form of pSP64T that contains KpnI & XhoI near BglII
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Sp6 expression for use in Xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BMP2-Vg1 dom neg
  • Alt name
    dnmVg1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1270
  • Mutation
    dominant negative version of BMP2-Vg1 chimera. The last 2 cysteines have been altered to a glycine and a serine (ECGCR -> EGGSR)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A dominant negative mutant version of pSP64T3 BMP2 pro Vg1.

See article for more cloning details.

The plasmid was linearized with EcoRI prior to in vitro synthesis of synthetic capped mRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T3 BMP2 pro dnmVg1 (DM#182) was a gift from Douglas Melton (Addgene plasmid # 15074 ; http://n2t.net/addgene:15074 ; RRID:Addgene_15074)

  • For your References section:

    Processed Vg1 protein is an axial mesoderm inducer in Xenopus. Thomsen GH, Melton DA. Cell. 1993 Aug 13. 74(3):433-41. 10.1016/0092-8674(93)80045-G PubMed 8348610
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