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Addgene

pCAGGS mutated Pdx1 (DM#233)
(Plasmid #15072)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15072 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pdx1
  • Species
    G. gallus (chicken)
  • Mutation
    DNA binding mutant

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutated Pdx-1 protein is unable to bind DNA; made by changing the leucine between helices 2 and 3 of the homeodomain into a proline.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS mutated Pdx1 (DM#233) was a gift from Douglas Melton (Addgene plasmid # 15072 ; http://n2t.net/addgene:15072 ; RRID:Addgene_15072)
  • For your References section:

    Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Grapin-Botton A, Majithia AR, Melton DA. Genes Dev. 2001 Feb 15. 15(4):444-54. 10.1101/gad.846001 PubMed 11230152