pCAGGS mutated Pdx1 (DM#233)
(Plasmid
#15072)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4800
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePdx1
-
SpeciesG. gallus (chicken)
-
MutationDNA binding mutant
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutated Pdx-1 protein is unable to bind DNA; made by changing the leucine between helices 2 and 3 of the homeodomain into a proline.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAGGS mutated Pdx1 (DM#233) was a gift from Douglas Melton (Addgene plasmid # 15072 ; http://n2t.net/addgene:15072 ; RRID:Addgene_15072) -
For your References section:
Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Grapin-Botton A, Majithia AR, Melton DA. Genes Dev. 2001 Feb 15. 15(4):444-54. 10.1101/gad.846001 PubMed 11230152