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Addgene

pSP70 Vg1 (DM#139)
(Plasmid #15070)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP70
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vg1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2300
  • Entrez Gene
    gdf1 (a.k.a. dvr-1, dvr1, gdf-1, vg1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BamHI to EcoRI fragment of T-Vg1-A. See author's map for additional information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP70 Vg1 (DM#139) was a gift from Douglas Melton (Addgene plasmid # 15070 ; http://n2t.net/addgene:15070 ; RRID:Addgene_15070)

  • For your References section:

    Localized synthesis of the Vg1 protein during early Xenopus development. Tannahill D, Melton DA. Development. 1989 Aug . 106(4):775-85. PubMed 2562668
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