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Addgene

pSP64T BMPR (DM#136)
(Plasmid #15068)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64TNE
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP receptor
  • Alt name
    BMPR
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1500
  • Entrez Gene
    bmpr1a (a.k.a. acvrlk3, alk-3, alk3, cd292, skr5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make mRNA for the full length BMP receptor. The clone contains no untranslated sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T BMPR (DM#136) was a gift from Douglas Melton (Addgene plasmid # 15068 ; http://n2t.net/addgene:15068 ; RRID:Addgene_15068)

  • For your References section:

    Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Graff JM, Thies RS, Song JJ, Celeste AJ, Melton DA. Cell. 1994 Oct 7. 79(1):169-79. 10.1016/0092-8674(94)90409-X PubMed 7522972
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