pSP64T tBMPR (DM#135)
(Plasmid
#15067)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP64T
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBMP receptor
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Alt nameBMPR
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SpeciesX. laevis (frog)
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Insert Size (bp)531
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MutationTruncated dominant negative form
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Entrez Genebmpr1a (a.k.a. acvrlk3, alk-3, alk3, cd292, skr5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make RNA for truncated dominant negative form of RU1. The truncation is at one amino acid past the transmembrane domain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64T tBMPR (DM#135) was a gift from Douglas Melton (Addgene plasmid # 15067 ; http://n2t.net/addgene:15067 ; RRID:Addgene_15067) -
For your References section:
Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Graff JM, Thies RS, Song JJ, Celeste AJ, Melton DA. Cell. 1994 Oct 7. 79(1):169-79. 10.1016/0092-8674(94)90409-X PubMed 7522972
Map uploaded by the depositor.