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Addgene

pBS BMPR (DM#137)
(Plasmid #15059)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15059 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript KS II +
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP receptor
  • Alt name
    BMPR
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2200
  • Entrez Gene
    bmpr1a (a.k.a. acvrlk3, alk-3, alk3, cd292, skr5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains entire ORF of BMP receptor. The insert cuts with EcoRV, HindIII, BglII, KpnI, BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS BMPR (DM#137) was a gift from Douglas Melton (Addgene plasmid # 15059 ; http://n2t.net/addgene:15059 ; RRID:Addgene_15059)
  • For your References section:

    Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Graff JM, Thies RS, Song JJ, Celeste AJ, Melton DA. Cell. 1994 Oct 7. 79(1):169-79. 10.1016/0092-8674(94)90409-X PubMed 7522972