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Addgene

pF5 Xfin (DM#126)
(Plasmid #15054)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP70
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2417
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xfin fragment
  • Alt name
    Xfin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1500
  • Entrez Gene
    znf208 (a.k.a. xfin, pmidp, znf95)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF5 Xfin (DM#126) was a gift from Douglas Melton (Addgene plasmid # 15054 ; http://n2t.net/addgene:15054 ; RRID:Addgene_15054)

  • For your References section:

    Xfin: an embryonic gene encoding a multifingered protein in Xenopus. Ruiz i Altaba A, Perry-O'Keefe H, Melton DA. EMBO J. 1987 Oct . 6(10):3065-70. PubMed 2826129
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