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Addgene

pSP64T Activin betaB (DM#108)
(Plasmid #15045)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Activin Beta b
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200
  • Entrez Gene
    inhbb (a.k.a. inhibin)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T Activin betaB (DM#108) was a gift from Douglas Melton (Addgene plasmid # 15045 ; http://n2t.net/addgene:15045 ; RRID:Addgene_15045)

  • For your References section:

    Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures. Thomsen G, Woolf T, Whitman M, Sokol S, Vaughan J, Vale W, Melton DA. Cell. 1990 Nov 2. 63(3):485-93. 10.1016/0092-8674(90)90445-K PubMed 2225062
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