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pSP64T XAR1 (DM#105)
(Plasmid #15044)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3260
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xenopus activin receptor 1
  • Alt name
    XAR1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1600
  • Entrez Gene
    xstk2 (a.k.a. MGC80923)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site SspI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORF of XAR1 in p64T to bypass possible translational regulation. This construct still has the 5' UTR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T XAR1 (DM#105) was a gift from Douglas Melton (Addgene plasmid # 15044 ; http://n2t.net/addgene:15044 ; RRID:Addgene_15044)

  • For your References section:

    A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos. Hemmati-Brivanlou A, Melton DA. Nature. 1992 Oct 15. 359(6396):609-14. 10.1038/359609a0 PubMed 1328888
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