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Addgene

pSP64T XAR1 DNM1 (DM#102)
(Plasmid #15042)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3260
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Xenopus activin receptor 1
  • Alt name
    XAR1
  • Species
    X. laevis (frog)
  • Mutation
    Dominant negative mutant
  • Entrez Gene
    xstk2 (a.k.a. MGC80923)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (destroyed during cloning)
  • 3′ cloning site SacII (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For expression in embryos. Note that this construct does not have a 5' UTR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64T XAR1 DNM1 (DM#102) was a gift from Douglas Melton (Addgene plasmid # 15042 ; http://n2t.net/addgene:15042 ; RRID:Addgene_15042)

  • For your References section:

    A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos. Hemmati-Brivanlou A, Melton DA. Nature. 1992 Oct 15. 359(6396):609-14. 10.1038/359609a0 PubMed 1328888
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