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Addgene

pCS3 xenopus Mixer (DM#180?)
(Plasmid #15038)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS300 (modified pCS2)
  • Backbone size w/o insert (bp) 4100
  • Vector type
    xenopus/mammalian/avian/zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mixer
  • Alt name
    Mix.3
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1450
  • Entrez Gene
    mixer (a.k.a. tMixer)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCS300 differs from pCS2 in that the polylinker II Not I site was moved to the polylinker I area and an Asc I and Pme I site were added to the polylinker II area.

You can use the CMV promoter in tissue culture cells or Xenopus embryos.

See the author's map for more cloning information. See the pdf attachment for information about making antisense probes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS3 xenopus Mixer (DM#180?) was a gift from Douglas Melton (Addgene plasmid # 15038 ; http://n2t.net/addgene:15038 ; RRID:Addgene_15038)

  • For your References section:

    Mixer, a homeobox gene required for endoderm development. Henry GL, Melton DA. Science. 1998 Jul 3. 281(5373):91-6. 10.1126/science.281.5373.91 PubMed 9651252
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