Hsp68-CreER(TM) (DM#264)
(Plasmid #15028)

• Depositing Lab: Douglas Melton
• Publication: Other Melton Lab plasmids (unpublished)

Backbone

• Vector backbone: modified pBluescript II KS
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression, Cre/Lox ; for making transgenic constructs

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre-ER(TM)
• Alt name: Cre-ER (TM)
• Insert Size (bp): 3700
• Tags / Fusion Proteins:
  • Hsp68 promoter (N terminal on insert)
  • polyA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cre-ER(TM) was a gift from Andy McMahon. Hsp68 was a gift from Maureen Gannon.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.

See Author's Map for more information.