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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression, Cre/Lox ; mouse transgenic
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNgn3 promoter
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Alt namepGD84
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SpeciesM. musculus (mouse)
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Insert Size (bp)7900
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Entrez GeneNeurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)
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Tag
/ Fusion Protein
- Cre (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMC-Cre was a gift from Andrew McMahon.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production.
See Author's Map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ngn3 Cre (DM#259) was a gift from Douglas Melton (Addgene plasmid # 15023 ; http://n2t.net/addgene:15023 ; RRID:Addgene_15023) -
For your References section:
Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Gu G, Dubauskaite J, Melton DA. Development. 2002 May . 129(10):2447-57. PubMed 11973276
Map uploaded by the depositor.