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Addgene

RCAS chick Pax6 (DM#226)
(Plasmid #15016)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS (A)
  • Backbone size w/o insert (bp) 11500
  • Vector type
    Retroviral ; avian

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pax6
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2000
  • Entrez Gene
    PAX6 (a.k.a. PAX-6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS chick Pax6 (DM#226) was a gift from Douglas Melton (Addgene plasmid # 15016 ; http://n2t.net/addgene:15016 ; RRID:Addgene_15016)

  • For your References section:

    Development of the pancreas in Xenopus laevis. Kelly OG, Melton DA. Dev Dyn. 2000 Aug . 218(4):615-27. 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1027>3.0.CO;2-8 PubMed 10906780
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