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Addgene

pBS xenopus Islet1 (DM#219)
(Plasmid #15015)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    islet1
  • Alt name
    Xislet1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS xenopus Islet1 (DM#219) was a gift from Douglas Melton (Addgene plasmid # 15015 ; http://n2t.net/addgene:15015 ; RRID:Addgene_15015)

  • For your References section:

    Development of the pancreas in Xenopus laevis. Kelly OG, Melton DA. Dev Dyn. 2000 Aug . 218(4):615-27. 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1027>3.0.CO;2-8 PubMed 10906780
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