pSP64TS porcine TGFbeta1 (DM#167)
(Plasmid #15011)

• Depositing Lab: Douglas Melton
• Publication: Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Backbone

• Vector backbone: pSP64TS
• Backbone size w/o insert (bp): 3000
• Vector type: Sp6 expression for use in Xenopus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TGFb type 1
• Alt name: TGFB1
• Alt name: TGF beta type I
• Species: porcine (Sus scrofa)
• Insert Size (bp): 1200
• Entrez Gene: TGFB1 (a.k.a. TGF-BETA-1)
• Tag / Fusion Protein:
  • Flag / HMK

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site.

See Author's Map for more information.