pTRACER LA
(Plasmid #15003)

• Depositing Lab: Raymond Birge
• Publication: Other Birge Lab plasmids (unpublished)

Backbone

• Vector backbone: pTracer-EF/V5-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5933
• Vector type: Mammalian Expression
• Selectable markers: GFP-Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LA
• Alt name: MFGE8
• Species: H. sapiens (human)
• Entrez Gene: MFGE8 (a.k.a. BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47)
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry