pCMV5 TrkA
(Plasmid #15002)

• Depositing Lab: Raymond Birge
• Publication: Yano et al J Neurosci Res. 2000 Feb 1. 59(3):356-64.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TrkA
• Alt name: Trk A
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: V289L
• Entrez Gene: NTRK1 (a.k.a. MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TrkA was originally from B Hempstead, and was generated in the lab of Dr. Moses Chao.
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mammalian expression plasmid for trkA was generated by subcloning of TrkA full-length cDNA into pCMV5 vector at the EcoRI site.