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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCX-EGFP
- Backbone size w/o insert (bp) 6182
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta5 integrin receptor
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Alt nameb5 integrin receptor
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SpeciesH. sapiens (human)
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Entrez GeneITGB5
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Tag
/ Fusion Protein
- IRES EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHuman beta5 cDNA provided by Dr. David Cheresh. pCX-EGFP provided by Dr. Hidesaburo Hanafusa.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length human beta5 expression vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCX-EGFP beta5 integrin receptor was a gift from Raymond Birge (Addgene plasmid # 14996 ; http://n2t.net/addgene:14996 ; RRID:Addgene_14996) -
For your References section:
alphavbeta5 integrin recruits the CrkII-Dock180-rac1 complex for phagocytosis of apoptotic cells. Albert ML, Kim JI, Birge RB. Nat Cell Biol. 2000 Dec . 2(12):899-905. 10.1038/35046549 PubMed 11146654