Skip to main content
Addgene

pDRIVE-CAG-hFX-HA
(Plasmid #14994)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDRIVE-CAG
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 6353
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human frataxin cDNA
  • Alt name
    hFX
  • Alt name
    FRX
  • Alt name
    frataxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    686
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • AU1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRIVE-CAG-hFX-HA was a gift from Michael Ristow (Addgene plasmid # 14994 ; http://n2t.net/addgene:14994 ; RRID:Addgene_14994)

  • For your References section:

    Activation of mitochondrial energy metabolism protects against cardiac failure. Schulz TJ, Westermann D, Isken F, Voigt A, Laube B, Thierbach R, Kuhlow D, Zarse K, Schomburg L, Pfeiffer AF, Tschope C, Ristow M. Aging (Albany NY). 2010 Nov;2(11):843-53. doi: 10.18632/aging.100234. 10.18632/aging.100234 PubMed 21084725
Related items:
Commonly requested with: