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Addgene

pBlue-hFX(T133E)-HA
(Plasmid #14990)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human frataxin cDNA
  • Alt name
    hFX
  • Alt name
    FRX
  • Alt name
    frataxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Mutation
    changed threonine 133 to glutamic acid
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • AU1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBlue-hFX(T133E)-HA was a gift from Michael Ristow (Addgene plasmid # 14990 ; http://n2t.net/addgene:14990 ; RRID:Addgene_14990)
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