pGL3-basic-hFX-promotor(2,5kb)
(Plasmid #14980)

• Depositing Lab: Michael Ristow
• Publication: Ristow lab plasmids - promotor (unpublished)

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human frataxin promoter fragment
• Alt name: frataxin
• Species: H. sapiens (human)
• Insert Size (bp): 2487
• Mutation: start codon for human frataxin gene has been mutated (ATG to AAG) to facilitate expression of reporter gene in vector; single XhoI site has been inserted after mutated start codon
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry