pGL3-basic-hFX-promotor(1,3kb)
(Plasmid
#14979)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman frataxin promoter fragment
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Alt namefrataxin
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SpeciesH. sapiens (human)
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Insert Size (bp)1353
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Mutationstart codon for human frataxin gene has been mutated (ATG to AAG) to facilitate expression of reporter gene in vector; single XhoI site has been inserted after mutated start codon
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Entrez GeneFXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-basic-hFX-promotor(1,3kb) was a gift from Michael Ristow (Addgene plasmid # 14979 ; http://n2t.net/addgene:14979 ; RRID:Addgene_14979)