Skip to main content
Addgene

pCINeo-hFX(I154F)-HA
(Plasmid #14977)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human frataxin cDNA
  • Alt name
    hFX
  • Alt name
    FRX
  • Alt name
    frataxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    690
  • Mutation
    changed isoleucine 154 to phenylalanine
  • Entrez Gene
    FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • AU1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo-hFX(I154F)-HA was a gift from Michael Ristow (Addgene plasmid # 14977 ; http://n2t.net/addgene:14977 ; RRID:Addgene_14977)

  • For your References section:

    Induction of oxidative metabolism by mitochondrial frataxin inhibits cancer growth: Otto Warburg revisited. Schulz TJ, Thierbach R, Voigt A, Drewes G, Mietzner B, Steinberg P, Pfeiffer AF, Ristow M. J Biol Chem. 2006 Jan 13. 281(2):977-81. 10.1074/jbc.M511064200 PubMed 16263703
Related items:
Commonly requested with: