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Addgene

pMSCV TbetaRII ecto
(Plasmid #14974)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV Puro
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TbetaRII ecto
  • Alt name
    TbetaR-II
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    530
  • Mutation
    ecto domain
  • Entrez Gene
    TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Soluble TbetaRII. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV TbetaRII ecto was a gift from Joan Massague (Addgene plasmid # 14974 ; http://n2t.net/addgene:14974 ; RRID:Addgene_14974)

  • For your References section:

    TGF-beta directly targets cytotoxic T cell functions during tumor evasion of immune surveillance. Thomas DA, Massague J. Cancer Cell. 2005 Nov . 8(5):369-80. 10.1016/j.ccr.2005.10.012 PubMed 16286245
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