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pLV-ATF5-PROX1-HNF1A
(Plasmid #149725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CmiR0001-MR03
  • Backbone manufacturer
    GeneCopoeia
  • Backbone size w/o insert (bp) 5784
  • Total vector size (bp) 11269
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATF5-PROX1-HNF1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5485
  • GenBank ID
    NM_001193646, NM_001270616, NM_000545
  • Entrez Gene
    ATF5 (a.k.a. ATFX, HMFN0395)
  • Promoter CMV
  • Tag / Fusion Protein
    • P2A, T2A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site Acc65I (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-ATF5-PROX1-HNF1A was a gift from Baisong Lu (Addgene plasmid # 149725 ; http://n2t.net/addgene:149725 ; RRID:Addgene_149725)

  • For your References section:

    3D scaffold-free microlivers with drug metabolic function generated by lineage-reprogrammed hepatocytes from human fibroblasts. Lu Z, Priya Rajan SA, Song Q, Zhao Y, Wan M, Aleman J, Skardal A, Bishop C, Atala A, Lu B. Biomaterials. 2021 Feb;269:120668. doi: 10.1016/j.biomaterials.2021.120668. Epub 2021 Jan 8. 10.1016/j.biomaterials.2021.120668 PubMed 33461059
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