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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-Myc
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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GenBank IDNM_002467.2 NP_002458.1
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS human c-Myc was a gift from Joan Massague (Addgene plasmid # 14971 ; http://n2t.net/addgene:14971 ; RRID:Addgene_14971) -
For your References section:
Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression. Siegel PM, Shu W, Massague J. J Biol Chem. 2003 Sep 12. 278(37):35444-50. 10.1074/jbc.M301413200 PubMed 12824180