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Addgene

pLenti-EF1α-IRES-EGFP-hMCIDAS
(Plasmid #149696)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1α-IRES-EGFP
  • Backbone size w/o insert (bp) 10500
  • Total vector size (bp) 11700
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MCIDAS
  • Alt name
    IDAS
  • Alt name
    MULTICILIN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    MCIDAS (a.k.a. CILD42, IDAS, MCI, MCIN)
  • Promoter EF1 alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer TTCTCAAGCCTCAGACAGTG
  • 3′ sequencing primer AAGCGGCTTCGGCCAGTAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF1α-IRES-EGFP-hMCIDAS was a gift from Ken-Ichi Takemaru (Addgene plasmid # 149696 ; http://n2t.net/addgene:149696 ; RRID:Addgene_149696)
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