pAAV-hSyn-ChrimsonR-LSSmOrange
(Plasmid
#149691)
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PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4672
- Total vector size (bp) 6481
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChrimsonR
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Insert Size (bp)1809
- Promoter hSyn
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Tag
/ Fusion Protein
- LSSmOrange (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer TGAGAGCGCAGTCGAGAGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-ChrimsonR-LSSmOrange was a gift from Robert Malenka (Addgene plasmid # 149691 ; http://n2t.net/addgene:149691 ; RRID:Addgene_149691) -
For your References section:
Selective filtering of excitatory inputs to nucleus accumbens by dopamine and serotonin. Christoffel DJ, Walsh JJ, Hoerbelt P, Heifets BD, Llorach P, Lopez RC, Ramakrishnan C, Deisseroth K, Malenka RC. Proc Natl Acad Sci U S A. 2021 Jun 15;118(24). pii: 2106648118. doi: 10.1073/pnas.2106648118. 10.1073/pnas.2106648118 PubMed 34103400