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Purpose(Empty Backbone) Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTA-Mob
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Backbone manufacturerStrand et al., 2014
- Backbone size (bp) 56450
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Modifications to backboneInsertion of yeast artificial chromosome elements (HIS3-CEN6-ARSH4 and URA3)
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Vector typeBacterial Expression, Synthetic Biology ; Conjugation helper plasmid
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Selectable markersURA3, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Escherichia coli EPI300
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTA-Mob 2.0 was a gift from Bogumil Karas (Addgene plasmid # 149662 ; http://n2t.net/addgene:149662 ; RRID:Addgene_149662) -
For your References section:
Trans-Kingdom Conjugation within Solid Media from Escherichia coli to Saccharomyces cerevisiae. Soltysiak MPM, Meaney RS, Hamadache S, Janakirama P, Edgell DR, Karas BJ. Int J Mol Sci. 2019 Oct 21;20(20). pii: ijms20205212. doi: 10.3390/ijms20205212. 10.3390/ijms20205212 PubMed 31640164
Map uploaded by the depositor.