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Addgene

pFA197
(Plasmid #149602)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149602 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFB262
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8000
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeGFP
  • Insert Size (bp)
    717
  • Promoter PcaO_1O8

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gaaattcgcttatttagaagtgtc
  • 3′ sequencing primer CTCCTTCCTTTTCGGTTAGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA197 was a gift from Michael Jensen (Addgene plasmid # 149602 ; http://n2t.net/addgene:149602 ; RRID:Addgene_149602)

  • For your References section:

    High-Resolution Scanning of Optimal Biosensor Reporter Promoters in Yeast. Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey D, Jensen MK, Keasling JD. ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 10.1021/acssynbio.9b00333. Epub 2020 Jan 31. 10.1021/acssynbio.9b00333 PubMed 31935067
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