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Addgene

pBbdCas9H(-10TC)_arr2
(Plasmid #149587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbdCas9H(-10TC)
  • Backbone manufacturer
    CJW lab
  • Modifications to backbone
    dCas9, lacI, arr2
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Rifampicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB 5-alpha F' Iq
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Promoter PflaB, PpQE30

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbdCas9H(-10TC)_arr2 was a gift from Christine Jacobs-Wagner (Addgene plasmid # 149587 ; http://n2t.net/addgene:149587 ; RRID:Addgene_149587)

  • For your References section:

    A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311
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