Skip to main content
Addgene

pCMV5 Flag-Smad2 (1-456)
(Plasmid #14958)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    Amino acids 1-456 (tail deletion)
  • Entrez Gene
    SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer HGHpA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag-Smad2 (1-456) was a gift from Joan Massague (Addgene plasmid # 14958 ; http://n2t.net/addgene:14958 ; RRID:Addgene_14958)

  • For your References section:

    Mutations increasing autoinhibition inactivate tumour suppressors Smad2 and Smad4. Hata A, Lo RS, Wotton D, Lagna G, Massague J. Nature. 1997 Jul 3. 388(6637):82-7. 10.1038/40424 PubMed 9214507
Related items:
Commonly requested with: