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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmad1 (AAVA)
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Alt nameSmad1
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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MutationThree Serine to Alanine mutations in the C-terminal sequence SSVS
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Entrez GeneSMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5 Flag-Smad1 (AAVA) was a gift from Joan Massague (Addgene plasmid # 14957 ; http://n2t.net/addgene:14957 ; RRID:Addgene_14957) -
For your References section:
The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase. Kretzschmar M, Liu F, Hata A, Doody J, Massague J. Genes Dev. 1997 Apr 15. 11(8):984-95. 10.1101/gad.11.8.984 PubMed 9136927
Map uploaded by the depositor.