Skip to main content
Addgene

pYPQ265H
(Plasmid #149554)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pNJB91
  • Total vector size (bp) 8331
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please allow 2 days to grow at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAID-ziSpyMacCas9(D10A)-UGI
  • Mutation
    D10A, R221K, N394K, Protospacer interacting domain form Streptococcus macacae

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ265H was a gift from Yiping Qi (Addgene plasmid # 149554 ; http://n2t.net/addgene:149554 ; RRID:Addgene_149554)

  • For your References section:

    Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences. Sretenovic S, Yin D, Levav A, Selengut JD, Mount SM, Qi Y. Plant Commun. 2020 Jul 22;2(2):100101. doi: 10.1016/j.xplc.2020.100101. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100101 PubMed 33898973
Related items: