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Addgene

pYPQ166-iSpyMac
(Plasmid #149553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ165
  • Total vector size (bp) 7751
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible ziSpyMacCas9 entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iSpyMacCas9
  • Alt name
    3xFLAG-NLS-ziSpyMacCas9-NLS
  • Mutation
    R221K, N394K, Protospacer interacting domain form Streptococcus macacae
  • Tags / Fusion Proteins
    • 3X FLAG, NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ166-iSpyMac was a gift from Yiping Qi (Addgene plasmid # 149553 ; http://n2t.net/addgene:149553 ; RRID:Addgene_149553)

  • For your References section:

    Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences. Sretenovic S, Yin D, Levav A, Selengut JD, Mount SM, Qi Y. Plant Commun. 2020 Jul 22;2(2):100101. doi: 10.1016/j.xplc.2020.100101. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100101 PubMed 33898973
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