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Addgene

pAct-PE2-HygroR
(Plasmid #149552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAct-GW-HygroR
  • Backbone manufacturer
    Justin Bosch
  • Vector type
    Insect Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2
  • Alt name
    Prime editor 2
  • Species
    Synthetic
  • Insert Size (bp)
    6354
  • Promoter Act5c

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GAGCATTGCGGCTGATAAGG
  • 3′ sequencing primer CCTCGACTTCTCCTCCTCCT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PE2 coding sequence is from pCMV-PE2 (Addgene 132775). David Liu lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct-PE2-HygroR was a gift from Norbert Perrimon (Addgene plasmid # 149552 ; http://n2t.net/addgene:149552 ; RRID:Addgene_149552)

  • For your References section:

    Precise genome engineering in Drosophila using prime editing. Bosch JA, Birchak G, Perrimon N. Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118. 10.1073/pnas.2021996118 PubMed 33443210
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