pUAS-PE2-attB
(Plasmid #149550)

• Purpose: Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.
• Depositing Lab: Norbert Perrimon
• Publication: Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118.

Backbone

• Vector backbone: pWALIUM10-roe
• Backbone manufacturer: Drosophila Genomics Resource Center
• Modifications to backbone: PE2 added by Gateway Cloning LR reaction.
• Vector type: Insect Expression, CRISPR
• Selectable markers: mini-white+

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2
• Alt name: Prime editor 2
• Species: Synthetic
• Insert Size (bp): 6354
• Promoter: UAS

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: accagcaaccaagtaaatcaac
• 3′ sequencing primer: TAATCGTGTGTGATGCCTACC

Resource Information

• Supplemental Documents:
  • UAS-PE2-attB.gbk
• A portion of this plasmid was derived from a plasmid made by: PE2 coding sequence PCR amplified from pCMV-PE2 (Addgene 132775). David Liu lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry