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Addgene

pCMV5 Flag-Smad1 (4SP/AP+AAVA)
(Plasmid #14955)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad1 (4SP/AP+AAVA)
  • Alt name
    Smad1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Mutation
    Four Serine to Alanine mutations in the linker region; three Serine to Alanine mutations in the C-terminal sequence SSVS
  • Entrez Gene
    SMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag-Smad1 (4SP/AP+AAVA) was a gift from Joan Massague (Addgene plasmid # 14955 ; http://n2t.net/addgene:14955 ; RRID:Addgene_14955)

  • For your References section:

    The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase. Kretzschmar M, Liu F, Hata A, Doody J, Massague J. Genes Dev. 1997 Apr 15. 11(8):984-95. 10.1101/gad.11.8.984 PubMed 9136927
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