pGBW-m4137386
(Plasmid #149540)

• Purpose: Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo virus (unpublished)

Backbone

• Vector backbone: AmpR gene, with start and stop codons
• Backbone size w/o insert (bp): 860
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 S (surface glycoprotein)
• Alt name: Spike protein
• Alt name: S protein
• Species: H. sapiens (human); Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 6619
• Mutation: wt
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI reference sequence: YP_009724390.1 . This plasmid contains an 18 or 19bp truncation in the CMV promoter and was synthesized for making pseudotyped VSV particles. It has been shown that truncations either help expression and /or capture by VSVdeltaG. See https://www.ncbi.nlm.nih.gov/pubmed/19057867 for original paper on VSV pseudotyping, and https://www.cell.com/cell/pdf/S0092-8674(20)30229-4.pdf on more recent efforts with SARS-CoV-2.