pGBW-m4136460
(Plasmid
#149532)
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PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 149532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepG9m-2 lacIQ ampR
- Backbone size w/o insert (bp) 3177
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 E (envelope)
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Alt nameenvelope protein
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Alt nameE protein
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SpeciesEscherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
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Insert Size (bp)221
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Mutationwt
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Entrez GeneE (a.k.a. GU280_gp04)
- Promoter Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ; Start Codon | ATG
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Tag
/ Fusion Protein
- 6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NCBI reference sequence: YP_009724392.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBW-m4136460 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 149532 ; http://n2t.net/addgene:149532 ; RRID:Addgene_149532)