pGBW-m4136458
(Plasmid #149531)

• Purpose: Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
• Depositing Labs: Ginkgo Bioworks, Benjie Chen
• Publication: SARS-CoV-2 protein E. coli expression constructs, tagged, for protein expression and/or purification (unpublished)

Backbone

• Vector backbone: pG9m-2 lacIQ ampR
• Backbone size w/o insert (bp): 3177
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 S (surface glycoprotein)
• Alt name: Spike protein
• Alt name: S protein
• Species: Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 3815
• Mutation: wt
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ; Start Codon | ATG
• Tag / Fusion Protein:
  • 6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable TEV (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GinkgoBioworks

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

NCBI reference sequence: YP_009724390.1