pJJiaDEST-APEX2-GAL9
(Plasmid
#149520)
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PurposeExpress APEX2-Galectin-9 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJJiaDEST-APEX2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLGALS9
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Alt namegalectin-9; gal9
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SpeciesH. sapiens (human)
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Insert Size (bp)972
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GenBank IDNP_002299.2
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Entrez GeneLGALS9 (a.k.a. HUAT, LGALS9A)
- Promoter CMV
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Tag
/ Fusion Protein
- APEX2 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJiaDEST-APEX2-GAL9 was a gift from Vojo Deretic (Addgene plasmid # 149520 ; http://n2t.net/addgene:149520 ; RRID:Addgene_149520) -
For your References section:
Galectins Control mTOR in Response to Endomembrane Damage. Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V. Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 10.1016/j.molcel.2018.03.009. 10.1016/j.molcel.2018.03.009 PubMed 29625033
