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Addgene

pJJiaDEST-APEX2-GAL3
(Plasmid #149518)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149518 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJJiaDEST-APEX2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LGALS3
  • Alt name
    galectin-3; gal3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    795
  • GenBank ID
    NP_001344607.1
  • Entrez Gene
    LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
  • Promoter CMV
  • Tag / Fusion Protein
    • APEX2 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJiaDEST-APEX2-GAL3 was a gift from Vojo Deretic (Addgene plasmid # 149518 ; http://n2t.net/addgene:149518 ; RRID:Addgene_149518)
  • For your References section:

    Galectins Control mTOR in Response to Endomembrane Damage. Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V. Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 10.1016/j.molcel.2018.03.009. 10.1016/j.molcel.2018.03.009 PubMed 29625033