pJJiaDEST-APEX2
(Plasmid
#149517)
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Purpose(Empty Backbone) N-termial APEX2 tag. Gateway destination vector for mammanlian expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3 APEX2-NES
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Backbone manufacturerAddgene Plasmid #49386
- Backbone size (bp) 6198
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Modifications to backboneremove the stop codon from APEX2 and insert Gateway ATTR casset at XhoI enzyme site
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG-APEX2 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJiaDEST-APEX2 was a gift from Vojo Deretic (Addgene plasmid # 149517 ; http://n2t.net/addgene:149517 ; RRID:Addgene_149517) -
For your References section:
Galectins Control mTOR in Response to Endomembrane Damage. Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V. Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 10.1016/j.molcel.2018.03.009. 10.1016/j.molcel.2018.03.009 PubMed 29625033
Map uploaded by the depositor.
