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prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant
(Plasmid #149507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149507 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    prk5
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression ; Transient Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MFSD12
  • Alt name
    C19orf28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1440
  • Mutation
    Codon Optimized Sequence, p.LL256-257AA
  • Entrez Gene
    MFSD12 (a.k.a. C19orf28, PP3501)
  • Promoter CMV
  • Tag / Fusion Protein
    • TEV-3XHA-3XFLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant was a gift from David Sabatini (Addgene plasmid # 149507 ; http://n2t.net/addgene:149507 ; RRID:Addgene_149507)

  • For your References section:

    MFSD12 mediates the import of cysteine into melanosomes and lysosomes. Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, Lewis CA, Sabatini DM. Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 10.1038/s41586-020-2937-x. 10.1038/s41586-020-2937-x PubMed 33208952
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