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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFlag-pRK5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRAS40
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Alt nameAKT1S1
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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Entrez GeneAKT1S1 (a.k.a. Lobe, PRAS40)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe cDNA for PRAS40 was PCR'd from a clone obtained from Origene.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5 Flag PRAS40 was a gift from David Sabatini (Addgene plasmid # 14950 ; http://n2t.net/addgene:14950 ; RRID:Addgene_14950) -
For your References section:
PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E, Carr SA, Sabatini DM. Mol Cell. 2007 Mar 23. 25(6):903-15. 10.1016/j.molcel.2007.03.003 PubMed 17386266